一个PCR相关的专业网站，内容很全
7 o7 x4 T V" p0 \http://www.pcrlinks.com/
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实验方法与步骤详解
5 ]: x2 v$ K p- `http://swjsx.ntmc.edu.cn/Protocol/home.htm , U; B0 c/ N4 j- [, M8 L$ b* B
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美国AXYgen公司
% b+ \9 t) r/ j- G$ Thttp://www.axygen.com/NewFiles/pcr.html 2 b+ \; J7 b- d+ M
3 P1 t# f" u5 x; Dhttp://www.alkami.com/
* L h8 F8 k8 Z0 G! @7 i/ FThis 158 page PCR manual covers the following topics : N) N9 x) d! A5 M7 {
PCR Primer design + I" W2 k% l: j
PCR Methods
4 H7 s& a& L" g" V2 `/ j- b1 aPCR Polymerases L y d6 a: |2 a$ n: ~. R
PCR Variables 7 P/ t9 E+ l2 |# d. q& C j
PCR Troubleshooting
6 U& x6 i% J& R6 a: i4 q- P" uSpecial PCR Topics 6 B. G7 A! ~* ? ?- j
Appendixes and a comprehensive Index
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2 \0 d- Y" U& a) m" Y% ABioGuide - PCR 9 W9 ~3 ~) f8 Q0 ]$ G+ e' |& K
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html . C9 p \( E, C e% M% C2 B
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。 0 a) K1 m" ~# p, l0 w2 k( D) J
包括：
+ K5 A8 U% K. Z& Z- S0 FWhat is PCR? , ]% _3 Y9 X; T1 f* y$ T# k7 T
What are some good reference books for PCR? - ~" w X; Y* t- B. H# a
How should I select a set of primers to use for PCR? : P/ I, o. C7 Z0 O' D4 _
Programs for designing PCR primers 8 y2 g7 A# \1 _
What is "Hot-start" PCR?
% B: x+ J: K/ a+ p' PWhat is AP-PCR or RAPD PCR? : t& R1 [9 K( _) z
What is "Touchdown" PCR? , s) s( ~/ e X
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?
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( }6 c0 E3 s0 Z. X8 u1 S+ s$ fA Look at PCR Cloning Tools ! x3 G6 V. U, R. x9 V
http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0 % ~; K* t, N* r+ i1 k
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PCR常见问题及回答
. v- |; E* I% U* V @8 X9 W) Chttp://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 * j" }* h3 @+ Q- \+ p) I; b3 `
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PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo
- U" c3 @/ L4 g: T% Oint文件非常好
) o# w* p7 \0 }http://www.med.sc.edu:85/pcr/realtime-home.htm
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$ j8 C2 s) U& A' L, D! HSYBR Green PCR . H2 K6 l& j- `' t
http://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#63878
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PCR QUICK GUIDE
, u7 v4 O& X* y" u. hhttp://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
6 j2 I! z* I \) k' b1 lhttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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http://www.wooster.edu/biology/courses/bio309/default.html
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PCR拼接
, ~# v Y" S; ehttp://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0
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9 f4 z% k2 k) x: y; x& {( p0 a知道蛋白如何寻找其氨基酸序列
4 @ c+ \; O. v& w$ }2 o) }& Q! mhttp://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
8 Y& R1 m0 |- ]3 T5 n, khttp://us.expasy.org/cgi-bin/niceprot.pl?P08253 ; s; \" W; Z; C* J/ @$ v
" T* v% r5 u- ], t* Z关于DNA的flash版和文字版
( |! O1 I; S( Z* a4 ]http://www.pbs.org/wgbh/nova/genome/dna.html#
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ABI Prism 7000 简要使用说明 : ~1 S9 h' L3 ]9 u+ V
http://www.genomultix.com/tech/7000.htm \$ C3 ~! V$ ]( p {
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好书推荐：Practical Protocols of Gene Engineering + o" N3 f) F, `
http://www.estarray.org/books/ - z3 x) H7 ?) {3 A3 {: A9 N& ~
. v8 z0 I* N4 [4 W4 `) Ghttp://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html: c2 A; T/ q* [/ `) ^) G
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Website dealing with cloning and molecular analysis of genes
+ D4 V+ ^0 M1 Y' y+ Lhttp://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm 7 }( S) `6 k4 L8 O8 q6 t6 z
Some really wonderful illustrations of the techniques of genetic engineering! ; z+ }' r' u; [! O1 R3 L& @
( e( @; x) z" b9 h X: S+ j# j' e yhttp://www.uic.edu/classes/phar/phar331/lecture6/
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0 s4 r. D3 D L+ Z4 J: [A truly amazing global resource page on cloning, stem cell research, and relat
5 s$ l/ L2 A+ m, W {2 u( } Ned ethical issues, including recent news articles and legislative updates 3 w: w @$ H/ g
http://genomics.phrma.org/cloning.html
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: \2 Z- r0 r" @' UAnimation and article on interspecies cloning 1 `6 x- P( T8 `) Q, I8 A
http://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c
$ j2 \5 l2 t* R" x& G: X$ sampbell/HotTopics/InterspeciesCloning.html # {' ` d) ?! ~$ X* g
From PBS...an animation that illustrates how stem cells are cultivated
* @, p# }8 `8 E8 y5 Lhttp://www.pbs.org/newshour/health/stem_cells.swf 5 |. ?/ Z& O4 g" X
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Animations illustrating the use of viruses as vectors for gene therapy . z6 u, X) ^: `5 @
http://www.technologyreview.com/articles/5940.htm ! U& P* Z8 X) V; u5 Q' c
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+ B! n' b1 m' U% F+ s DAnimations of techniques used for cloning mammals, as well as a create-your-ow3 H" {& @( [5 M9 T% P; q y
n-clone link
# j1 y1 c3 h0 P9 g1 v0 \! I' ~http://library.thinkquest.org/24355/data/details/media/index.html
% H9 u6 g5 F, e( s3 iAnother animation of PCR
1 L& I* r) D# V" `http://www.people.virginia.edu/~rjh9u/pcranim.html 2 G$ k( V2 e" r# p$ e# H) W) T4 O, i% B `
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) L$ R' ` a; a: EAnimations of restriction digests and gel electrophoresis
: X2 ~- i% H- m+ v7 v5 Ehttp://www.biology.ucsc.edu/classes/bio20L/animate 8 i% {8 d5 D [: U; Y% l/ a6 t
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Gel electrophoresis techniques and simulations * z( ]# J* c$ t$ z& {5 v
A wonderful website that includes links to a virtual lab on agarose gel electr6 h! P) l& |" d9 N
ophoresis:
2 Y l7 u5 o" j2 {+ s8 W1 thttp://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/
) m, [8 F( Z: k# r. I* s% ~5 mA Massachusetts Institute of Technology website dealing with Southerns, Northe0 O& E. A! |* P7 t$ p
rns, Westerns, and cloning:
( t8 l# T6 n9 u6 X! j5 Zhttp://web.mit.edu/esgbio/www/rdna/rdna.html ?$ P' X( p3 N3 o$ W5 ^
2-D gel electrophoresis of proteins:
# d/ B; z4 F$ t0 Z7 r* t7 khttp://www.fitzscipress.com/Chap05.pdf
9 M3 a! Z2 f, l8 }- LA website containing lots of links to electrophoresis techniques and simulatio1 |( `3 q) j" G+ c# }* o) k6 y
ns:
! u% o F `3 o. Whttp://biobenchelper.hypermart.net/pr/electrophoresis.htm
, Z% M4 D# B0 u1 [& [Want to learn more about chemical bonds? Check out this website:
6 l) i3 V& s# N; T4 t1 lhttp://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
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多个录象-多个著名公司
) t! U9 H2 B" W3 c$ Khttp://www.biocompare.com/video.asp
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; j! N0 f6 z: A" c; F0 ]- }( NPCR和 RFLP动画片
+ Y; N" F8 ?, e: ]" |4 shttp://medtech.cls.msu.edu/ISL/immunology/pcr.htm
; \6 ^; r, E8 _" |5 _6 H$ Shttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm 5 m7 \: w/ V {
& [' t, W3 [5 `redasoft visual cloning 2000 patch
/ [# u6 q7 t3 ^3 Q# M$ ~http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0
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分子生物学屏幕保护程序下载 3 l8 ]% x8 O9 _% j* o$ T' ?5 H& g
pcr ; j- `- ?, m% H& l' o- I
http://biosoft.biosino.org/down/PCR.exe
0 G. h4 B3 E! k) _3 B: O6 ^8 l蛋白 # x; Q( f4 _# h5 n* L
http://biosoft.biosion.org/down/ssaver.zip 8 K; `$ ^# q3 I4 ?8 j$ b
amavi100 " l K! `7 x6 I5 }
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip 1 b- c* T9 c5 G: f1 N; n
dna旋转 4 a! _) ]+ u/ F! x W
http://www.rocketdownload.com/dnload/dna.exe 9 ]- n5 x' z6 u# }$ g
ddnabase 5 j G$ w8 M# t
http://www.rocketdownload.com/dnload/ddnabase.exe
; n+ M* t: B' fcyclovir
4 f& m; M1 ~% P- ^/ T& |http://mama.indstate.edu/pfaffle/software/cyclovir.exe
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% K4 d, E5 p" I; q( Uhttp://biosoft.biosino.org/down/source.exe ) Q) A1 o# ~, O8 |1 f1 K
http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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